Friend of African Studies Institute Current research efforts focus on improvement of cell culture systems for the isolation of viruses and on the molecular epidemiology of canine parvovirus. Although new molecular detection methods have rendered the detection of many viruses much faster than previously, the ability to culture viruses remains a necessity, especially for emerging and re-emerging viruses. When a new virus is isolated and amplified in cell culture, the isolate can be used for further characterization as well as for developing new vaccines and new diagnostic assays. Because virus isolation in cell culture is expensive, the goal of my current research is to determine ways of reducing the cost and time of virus isolation by studying the effect of short-term storage of cells at refrigerator temperatures on their susceptibility to virus infections and their usefulness in cell-based diagnostic tests. Canine parvovirus emerged in the late 1970s and has since become the single most important virus infecting dogs worldwide. There is evidence that the virus has continued to evolve since its emergence and at least three distinct antigenic variants are currently known to occur worldwide. Continuous evolution of the virus means that existing diagnostic tests and vaccines might need to be reformulated periodically to remain effective against current variants of the virus. In the United States, only two of the three known antigenic variants have been described. The goal of my research is to determine the current occurrence and distribution of various antigenic variants of canine parvovirus in the U.S. This information will be useful to vaccine and diagnostic kit manufacturers. Research Research Interests: Development and improvement of conventional and molecular diagnostic methods for existing and emerging viruses of terrestrial and marine mammals Awards, Honors and Recognitions Of note: Selected Publications Search PubMed for “saliki jt” Woolums AR, Berghaus RD, Berghaus LJ, Ellis RW, Pence ME, Saliki JT, Hurley, KA, Galland KL, Burdett WW, Nordstrom ST, Hurley DJ. Effect of calf age and administration route of initial multivalent modified-live virus vaccine on humoral and cell-mediated immune responses following subsequent administration of a booster vaccination at weaning in beef calves. Am J Vet Res. 2013 Feb;74(2):343-54. Anthony SJ, St Leger JA, Pugliares K, Ip HS, Chan JM, Carpenter ZW, Navarrete-Macias I, Sanchez-Leon M, Saliki JT, Pedersen J, Karesh W, Daszak P, Rabadan R, Rowles T, Lipkin WI. Emergence of fatal avian influenza in New England harbor seals. MBio. 2012 Jul 31;3(4):e00166-12. Bossart GD, Romano TA, Peden-Adams MM, Schaefer A, McCulloch S, Goldstein JD, Rice CD, Saliki JT, Fair PA, Reif JS. Clinicoimmunopathologic findings in Atlantic bottlenose dolphins Tursiops truncatus with positive cetacean morbillivirus antibody titers. Dis Aquat Organ. 2011 Dec 6;97(2):103-12. Stone BM, Blyde DJ, Saliki JT, Morton JM. Morbillivirus infection in live stranded, injured, trapped, and captive cetaceans in southeastern Queensland and northern New South Wales, Australia. J Wildl Dis. 2012 Jan;48(1):47-55. Stone BM, Blyde DJ, Saliki JT, Blas-Machado U, Bingham J, Hyatt A, Wang J, Payne J, Crameri S. Fatal cetacean morbillivirus infection in an Australian offshore bottlenose dolphin (Tursiops truncatus). Aust Vet J. 201, Nov;89(11):452-7. Woldemeskel M, Liggett A, Ilha M, Saliki JT, Johnson LP. Canine parvovirus-2b-associated erythema multiforme in a litter of English Setter dogs. J Vet Diagn Invest. 2011 May;23(3):576-80. Blas-Machado U, Saliki JT, Sánchez S, Brown CC, Zhang J, Keys D, Woolums A, Harvey SB. Pathogenesis of a bovine enterovirus-1 isolate in experimentally infected calves. Vet Pathol. 2011 Nov; 48(6):1075-84. Bossart GD, Reif JS, Schaefer AM, Goldstein J, Fair PA, Saliki JT. Morbillivirus infection in free-ranging Atlantic bottlenose dolphins (Tursiops truncatus) from the Southeastern United States: seroepidemiologic and pathologic evidence of subclinical infection. Vet Microbiol. 2010 Jul 14;143(2-4):160-6. Nollens HH, Rivera R, Palacios G, Wellehan JF, Saliki JT, Caseltine SL, Smith CR, Jensen ED, Hui J, Lipkin WI, Yochem PK, Wells RS, St Leger J, Venn-Watson S. New recognition of Enterovirus infections in bottlenose dolphins (Tursiops truncatus). Vet Microbiol. 2009 Oct 20;139(1-2):170-5. Fulton RW, Blood KS, Panciera RJ, Payton ME, Ridpath JF, Confer AW, Saliki JT, Burge LT, Welsh RD, Johnson BJ, Reck A. Lung pathology and infectious agents in fatal feedlot pneumonias and relationship with mortality, disease onset, and treatments. J Vet Diagn Invest. 2009 Jul;21(4):464-77.